![]() ![]() Les concentrations de chlorophylle a dans l'acétone estimées par une méthode spectrophotométrique (utilisant le rapport d'acidification) s'accordent avec celles estimés par HPLC. Grâce à une colonne en phase inversée, il est possible de diriger l'injection d'extrait préparé d'une manière conventionnelle (90% d'acétone ou d'autres mélanges de solvants contenant de l'eau). Les auteurs ont mis au point une méthode fondée sur la chromatographie en phase liquide à haute pression (HPLC) avec détecteur fluométrique pour le dosage des chlorophylles a et b, et de leurs phéophytines dans les sédiments. Key words: chlorophyll a, chlorophyll b, pheophytins, high-pressure liquid chromatography, sediments, algae, epipelic, biomass, primary productivity, suspended particulate matter Résumé ![]() ![]() The method is sensitive, rapid (14 min per sample), probably compatible with a variety of solvent mixtures, and can be automated for processing large numbers of samples. Estimates of concentrations of chlorophyll b and pheophytins a and b can be made by the HPLC method even in the presence of degradation products other than pheophytins that would interfere with the determination by spectrophotometry or fluorometry. Chlorophyll a concentrations in sediments estimated by fluorometry were sometimes quite different from those estimated by HPLC or spectrophotometry. Concentrations of chlorophyll a in acetone estimated by a spectrophotometric method (using the acidification ratio) agreed with those estimated by HPLC. A reversed-phase column allowed direct injection of extracts prepared in the conventional manner (90% acetone or other solvent mixtures containing water). We have developed a method that uses high-pressure liquid chromatography (HPLC) with fluorometric detector for the determination of concentrations of chlorophylls a and b and their pheophytins in sediments. ![]()
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